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Iminodiacetic acid is usually prepared on-site, such as by reaction of chloroacetic acid with ammonia and calcium hydroxide to produce the calcium iminodiacetate salt and then acidification of the product.

The chloroacetic acid approach is less efficient than other iminodiacetic acid approaches, owing to the production of calcium chloride waste and decreased yield.

The original synthetic approach to glyphosate involved the reaction of phosphorus trichloride with formaldehyde followed by hydrolysis to yield a phosphonate.

Glycine is then reacted with this phosphonate to yield glyphosate, and its name is taken as a contraction of the compounds used in this synthesis - viz. Two main approaches are used to synthesize glyphosate industrially.

The development of glyphosate resistance in weed species is emerging as a costly problem.

While glyphosate and formulations such as Roundup have been approved by regulatory bodies worldwide, concerns about their effects on humans and the environment persist.

Both the phosphonic acid and carboxylic acid moieties can be ionised and the amine group can be protonated and the substance exists as a series of zwitterions.

Glyphosate is soluble in water to 12 g/l at room temperature.

The European Chemicals Agency (ECHA) maintained their current classification of glyphosate as a substance causing serious eye damage and as a substance toxic to aquatic life, but did not find evidence implicating it to be a carcinogen, a mutagen, toxic to reproduction, nor toxic to specific organs.X-ray crystallographic studies of glyphosate and EPSPS show that glyphosate functions by occupying the binding site of the phosphoenolpyruvate, mimicking an intermediate state of the ternary enzyme–substrate complex.Glyphosate is readily degraded by soil microbes to aminomethylphosphonic acid (AMPA, which like glyphosate strongly adsorbs to soil solids and is thus unlikely to leach to groundwater).It is used to kill weeds, especially annual broadleaf weeds and grasses that compete with crops.It was discovered to be an herbicide by Monsanto chemist John E. Monsanto brought it to market in 1974 under the trade name Roundup, and Monsanto's last commercially relevant United States patent expired in 2000.Somewhat later, glyphosate was independently discovered at Monsanto in 1970.